Clinical Microbiology
Proficiency Scale
1 =
No Experience
2 =
Need Training
3 =
Able to perform with supervision
4 =
Able to perform independently
| Orientation and Lab Safety | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Review the laboratory's fire safety plan. | |||||
| Locate Personal Protective equipment and MSDS. | |||||
| Understand Universal Precautions for microbiology. |
| Labeling & Specimen ID | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Label specimens according to institutional policies. |
| Specimen Set Up & Incubation | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Select proper media for specimens including plated media and broth media. | |||||
| Understand specimen collection & rejection criteria. | |||||
| Incubate specimens properly. |
| Inoculation | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Demonstrate plate streaking for isolation & quantitative streaking for urines. |
| Quality Control | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Perform quality control procedures in accordance with institutional policies for new media, reagents, and stock culture organisms. | |||||
| Understand documentation & actions taken when results are not in acceptable limits. |
| Gram Staining | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Practice performing Gram stains until proficient. | |||||
| Evaluate grams stains, including sputum samples, wounds, genital samples, and positive blood cultures until proficient. |
| Evaluation of Primary Cultures | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Evaluate cultures to recognize what is normal flora and what is significant. | |||||
| Evaluate throat cultures & select next course of action. | |||||
| Evaluate urine cultures to decide when susceptibility testing is warranted. | |||||
| Evaluate vaginal cultures to recognize what is normal flora and what is significant. | |||||
| Evaluate stool cultures to recognize what is normal and what to process further. | |||||
| Evaluate body fluid cultures for pathogens. | |||||
| Evaluate wound cultures to recognize what is significant -select next course of action. | |||||
| Evaluate respiratory cultures, including sputum cultures. Recognize normal resp. flora & significant pathogens. |
| Blood Culture Processing | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Demonstrate procedure for processing positive blood cultures including subcultures, Gram stains, and proper reporting of results. |
| Primary ID of Organisms | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Recognize Streptococcus species & perform tests to classify them according to the Lancefield classification scheme. | |||||
| Recognize & perform tests to identify Staphylococcus species. | |||||
| Identify coagulase negative Staphylococcus species. | |||||
| Recognize and identify Haemophilus species. | |||||
| Recognize and identify Neisseria species. | |||||
| Recognize and perform biochemical tests to ID GNBs, including lactose fermenters, nonfermenters, and other miscellaneous GNBs. | |||||
| Recognize and identify Campylobacter species. |
| Interpretation and Acceptance of Results | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Discuss recording, reporting, and documenting results. | |||||
| Discuss which organisms are reportable to the State Health Department. |
| Affective Objectives - demonstrates honesty by: | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Maintaining strict patient confidentiality. | |||||
| Accepting control values only when within acceptable limits. | |||||
| Performing and documenting daily & weekly maintenance procedures, preventative maintenance, temperature checks, etc. | |||||
| Completing all procedures in adherence to laboratory SOPs, taking no shortcuts or unauthorized modifications of procedure. |
| Personal Interactive Skills - demonstrates proper professional behavior by: | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Working with co-workers in a positive manner, promoting productive workflow. | |||||
| Refraining from making statements or actions that represent sexual, ethnic, racial, or homophobic harassment. | |||||
| Willingly and consistently using appropriate personal safety devices when handling caustic, infectious, or hazardous materials. | |||||
| Completing all required tasks and remaining in the work area when scheduled. | |||||
| Being punctual whenever scheduled. | |||||
| Adhering to current dress and appearance in the laboratory setting. | |||||
| Cleaning the work area when leaving the laboratory, returning supplies to appropriate storage location, & disinfecting all work areas used by the student. |
| Professional Responsibility - demonstrates appropriate professional affective behavior by: | Rating Stars (Click) | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| Correctly reporting all patient test values, as well as recognizing and correctly reporting all patient critical test values. | |||||
| Resolving discrepancies in specimen labeling, handling, or collection before reporting patient results. |